The Afghan president has ordered US special forces to leave Wardak province within two weeks.
The decision was being taken due to allegations of disappearances and torture by Afghans considered to be part of US special forces, said a spokesman for Hamid Karzai.
The strategically significant, central province of Wardak has been the recent focus of counter-insurgency operations.
A US statement said it took all allegations of misconduct seriously.
But the US could not comment specifically on this latest development "until we have had a chance to speak with senior government officials", the statement by a spokesman for US special forces said.
This could cause a shitstorm.