Ankara contacted the US ambassador on Wednesday over comments the foreign ministry called "unacceptable".
A US military spokesman had expressed hopes on Tuesday that, rather than see Turkey pursue Kurdish fighters, "all parties involved are going to stop shooting at each other and focus" on IS.
On Wednesday, Russia added its voice, with the foreign affairs ministry calling on Turkey to avoid strikes in Syria on opposition and ethnic groups fighting Islamic State, including Syrian Kurds.
"Turkey is a sovereign state, it is a legitimate state," said Turkey's EU Affairs Minister Omer Celik.
"To suggest it is on a par with a terrorist organisation and suggest there are talks between them, that a deal has been reached between them, this is unacceptable."
Prime Minister Binali Yildirim said on Wednesday that
"operations will continue until all terrorist elements have been neutralised, until all threats to our borders, our lands and our citizens are completely over".