Originally Posted by
FlawlessSoul
Some of the crew can be recruited again, some as party members (Tali, Liara, Garrus, Ash/Kaidan, EDI) or assets for the war (Zaeed, Kasumi, Miranda, Samara, Grunt, Jack, Jacob, Wrex (ME1), Morinth, Thane, Mordin, Legion)., but they may die during the main story depending on your actions in ME2 and ME3. Mordin, as an example, will not die if Wrex is dead and Maelon's data on the genophage was destroyed. This would actually result in the Salarians, Krogan [I]and[/I] Mordin joining the final fight. Some crew will join you, on the Normandy, for a brief time.
ME2 non-party crew: (Kelly will be on the citadel, but won't return. The engineers Donnelly and Daniels can be pardoned by a Spectre (you) and return to the Normandy. Doctor Chakwas can be convinced to return to the Normandy, as well. Joker just comes with the ship.)